Author

N. K. Karpowich

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 2

Signature Assays, Organisms & Methods

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Organism:E. coli· 2 Research Focus:cell signaling & trafficking· 1 Approach:experimental assay· 1 Assay:fluorescence / live-cell microscopy· 1 Approach:microscopy & imaging analysis· 1

Frequent Co-Authors

C. D. Kinz-Thompson· 2 shared J. E. Ramos· 2 shared J. F. Hunt· 2 shared J. Kim· 2 shared K. Leng· 2 shared L. Zhu· 2 shared R. L. Gonzalez· 2 shared

Activity by Year

2022-2022 · peak 2/yr

Co-Author Collaboration Network

7 top collaborators · 21 mutual co-author links

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2 shared papersBiophysics

C. D. Kinz-Thompson

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2 papers

Recent·Most cited

Mechanistic implications of the interaction of the soluble substrate-binding protein with a type II ABC importer

L. Zhu, J. Kim, K. Leng, J. E. Ramos, C. D. Kinz-Thompson, N. K. Karpowich, R. L. Gonzalez, J. F. Hunt

The paper investigates the interaction kinetics of the SBP with a type II ABC importer using FRET, revealing a locked complex formation that requires ATP hydrolysis and is inhibited by ATP or B12 binding.

bioRxiv · Dec 2, 2022 · PreprintBiophysics

Realtime observation of ATP-driven single B12 molecule translocationthrough BtuCD-F

L. Zhu, J. Kim, K. Leng, J. E. Ramos, C. D. Kinz-Thompson, N. K. Karpowich, R. L. Gonzalez, J. F. Hunt

This study uses smFRET to observe real-time ATP-driven translocation of B12 through the E. coli BtuCD-F transporter, revealing two sequential conformational changes.

bioRxiv · Dec 2, 2022 · PreprintBiophysics