Multiplexed CRISPR/Cas9 mutagenesis of rice PSBS1 non-coding sequences for transgene-free overexpression
D. Patel-Tupper, A. Kelikian, A. Leipertz, N. Maryn, M. Tjahjadi, N. G. Karavolias, M.-J. Cho, K. K. Niyogi
This study uses CRISPR/Cas9 to generate transgene-free overexpression alleles of rice PSBS1, showing increased protein abundance and improved water-use efficiency.
bioRxiv · Oct 20, 2023 · PreprintPlant Biology