N. Sinsuebphon

in vivo quantitative FRET small animal imaging: intensity versus lifetime-based FRET

This paper compares intensity-based and fluorescence lifetime-based FRET methods for in vivo small animal imaging, showing that lifetime-based FRET is more efficient and accurate for quantifying drug-target engagement.

Multiplexed Non-invasive in vivo Imaging to Assess Metabolism and Receptor Engagement in Tumor Xenografts

This paper develops a multiplexed imaging method to simultaneously assess tumor metabolism and receptor engagement in vivo, revealing an inverse relationship between metabolic activity and drug delivery efficacy.

Ultra-fast fit-free analysis of complex fluorescence lifetime imaging via deep learning

This paper introduces FLI-Net, a deep learning model that enables ultra-fast, fit-free analysis of fluorescence lifetime imaging data across multiple spectral ranges and acquisition technologies.

In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging

The paper introduces a method using phasor analysis and NIR dyes to quantify fluorescence lifetime and stoichiometry in vitro and in vivo, demonstrating its application to FRET and pharmacokinetics studies.

Quantitative Imaging of Receptor-Ligand Engagement in Intact Live Animals

This study develops MFLI-FRET to quantify receptor-ligand engagement in live animals, showing it correlates with intracellular ligand binding rather than ex vivo receptor expression.