Deep learning-based codon optimization with large-scale synonymous variant datasets enables generalized tunable protein expression
David A. Constant, Jahir M. Gutierrez, Anand V. Sastry, Rebecca Viazzo, Nicholas R. Smith, Jubair Hossain, David A. Spencer, Hayley Carter, Abigail B. Ventura, Michael T. M. Louie, Christa Kohnert, Rebecca Consbruck, Joshua Bennett, Kenneth A. Crawford, John M. Sutton, , Miles W. Gander
The paper develops deep learning models trained on synonymous variant datasets to predict and optimize protein expression in E. coli.
bioRxiv · Feb 11, 2023 · PreprintSynthetic Biology