Engineering and characterisation of AAVS1 FRT cell lines for inducible and dose-dependent gene expression
Maria Portillo Malumbres, Lachlan Ellingboe, Annalisa Roberti…·Norman E Davey
This paper develops a stable RPE1 cell line with inducible and dose-dependent transgene expression using CRISPR/Cas9 and FRT/Tet-On systems, validated through multi-omics analysis.