Biological Plasticity Rescues Target Activity in CRISPR Knockouts
A. H. Smits, F. Ziebell, G. Joberty, N. Zinn, W. F. Mueller, S. Clauder-Münster, D. Eberhard, M. Fälth Savitski, P. Grandi, P. Jakob, A.-M. Michon, H. Sun, K. Tessmer, T. Bürckstümmer, M. Bantscheff, , W. Huber
The paper quantifies residual protein expression in CRISPR-Cas9 knockouts using RNA-seq and SPS-MS3, identifying mechanisms of partial protein preservation and functional retention.
bioRxiv · Jul 26, 2019Bioengineering