Targeted mutagenesis of Medicago truncatula Nodule-specific Cysteine-rich (NCR) genes using the Agrobacterium rhizogenes-mediated CRISPR/Cas9 system
B. Gungor, J. B. Biro, A. Domonkos, B. Horvath, P. Kalo
This study uses CRISPR/Cas9 to knockout specific NCR genes in Medicago truncatula and shows that NCR068, NCR089, NCR128, and NCR161 are not essential for symbiosis with Sinorhizobium species.
bioRxiv · Aug 23, 2023Plant Biology