Localising elements in single-particle reconstructions by REEL-EM: Reconstructed Electron Energy-Loss - Elemental Mapping
O. Pfeil-Gardiner, H. V. D. Rosa, D. Riedel, Y. S. Chen, D. Lorks, P. Kukelhan, M. Linck, H. Muller, F. Van Petegem, B. J. Murphy
This paper introduces REEL-EM, a method combining EELS and single-particle image processing to enable elemental mapping in cryo-preserved macromolecular complexes, demonstrating reliable localization of abundant elements.
bioRxiv · Jan 18, 2024Biophysics