F. Van Petegem

Localising elements in single-particle reconstructions by REEL-EM: Reconstructed Electron Energy-Loss - Elemental Mapping

This paper introduces REEL-EM, a method combining EELS and single-particle image processing to enable elemental mapping in cryo-preserved macromolecular complexes, demonstrating reliable localization of abundant elements.

Propofol directly binds and inhibits skeletal muscle ryanodine receptor 1 (RyR1)

This study shows propofol directly binds and inhibits RyR1, reducing Ca2+ release and potentially protecting against malignant hyperthermia.

Crystallographic, kinetic, and calorimetric investigation of PKA interactions with L-type calcium channels and Rad GTPase

This study identifies two tiers of PKA substrate sites in CaV1.2 and Rad, showing that Ser1981 and Ser25/Ser272 are preferentially phosphorylated, with structural insights into PKA's substrate recognition.

RyR2 Binding of an Antiarrhythmic Cyclic Depsipeptide Mapped Using Confocal Fluorescence Lifetime Detection of FRET

The paper maps the binding site of ent-verticilide on RyR2 using FRET and computational methods, identifying two potential loci within the RyR2 structure.

Structures of PKA-phospholamban complexes reveal a mechanism of familial dilated cardiomyopathy

This study reveals how PLN mutations reduce phosphorylation by altering conformation and PKA interactions, contributing to DCM.

The effect of magnesium on calcium binding to cardiac troponin C related hypertrophic cardiomyopathy mutants

This study investigates how magnesium affects calcium binding to cardiac troponin C mutants linked to hypertrophic cardiomyopathy, showing that Mg2+ competes with Ca2+ and modifies binding affinity.

Cardiac RyR N-terminal region biosensors for FRET-based high-throughput screening

This study develops FRET-based biosensors to screen for RyR modulators, identifying novel inhibitors and activators with therapeutic potential.

Interleukin-10 and Small Molecule SHIP1 Allosteric Regulators Trigger Anti-Inflammatory Effects Through SHIP1/STAT3 Complexes

The paper shows IL10 induces a SHIP1/STAT3 complex in macrophages, and SHIP1 activators mimic IL10's anti-inflammatory effects in colitis.