Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter
P. P. M. Mathiassen, A. K. Menon, T. Guenther Pomorski
The paper develops a fluorescence microscopy assay to measure scramblase activity in yeast ER proteins reconstituted into giant unilamellar vesicles using a photostable lipid reporter.
bioRxiv · Apr 1, 2021Biochemistry