Cloning of Human ABCB11 Gene in E. coli required the removal of an Intragenic Pribnow-Schaller Box before its Insertion into Genomic Safe Harbor AAVS1 Site using CRISPR Cas9
N. Vats, M. G. Sanal, S. K. Venugopal, P. Taneja, S. K. Sarin
The paper inserts the human ABCB11 gene into the AAVS1 site in HEK 293T cells using CRISPR-Cas9, but reports low homologous recombination efficiency.
bioRxiv · Sep 5, 2020Cell Biology