Experimental characterization of in silico red-shift predicted iLOVL470T/Q489K and iLOVV392K/F410V/A426S mutants
P. Wehler, D. Armbruster, A. Guenter, E. Schleicher, B. Di Ventura, M. A. Oeztuerk
The paper experimentally tests two computationally predicted red-shifted iLOV mutants, finding they have reduced brightness, blue shifts, and low expression, limiting their imaging utility.
bioRxiv · Mar 25, 2021Biophysics