P. Reinke

Gene editing of CD3 epsilon gene to redirect regulatory T cells for adoptive T cell transfer

This paper develops a non-viral CRISPR-Cas12a method to redirect Tregs with CARs by integrating an antigen-binding domain into the CD3 epsilon gene, showing improved antigen-specific function and graft protection in a mouse model.

Integration of ζ-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells

This paper develops a method to integrate ζ-deficient CARs into the CD3ζ gene to create functional CAR-T and CAR-NK cells with improved safety and efficacy for off-the-shelf therapies.

Matching or genetic engineering of HLA Class I and II facilitates successful allogeneic 'off-the-shelf' regulatory T cell therapy

This study shows that matching or engineering HLA-I and HLA-II improves the efficacy of allogeneic regulatory T cells for immunoregulation.

Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells

This paper combines CRISPR nucleases to simultaneously knock-in and base edit CAR T cells, reducing translocations to 1.5% and improving safety.

Human iPSC-derived renal cells change their immunogenic properties during maturation: Implications for regenerative therapies

This study shows that hiPSC-derived renal cells become less immunogenic as they mature, with implications for improving immune tolerance in regenerative therapies.

Fast, efficient and virus-free generation of TRAC-replaced CAR T cells

This paper presents a virus-free method to generate CAR T cells by inserting CARs into the TRAC locus using CRISPR-Cas and HDR, achieving up to 68% TCR-to-CAR replacement.

Sex-associated differences in cytomegalovirus prevention: Prophylactic strategy is associated with a strong kidney function impairment in female renal transplant patients

This study compares prophylactic and pre-emptive strategies for preventing CMV syndrome in renal transplant patients, finding that the prophylactic strategy impairs kidney function in females.

BKV clearance time correlates with exhaustion state and T-cell receptor repertoire shape of BKV-specific T-cells in renal transplant patients

The paper investigates factors affecting BKV clearance in kidney transplant patients, finding that T-cell receptor diversity and exhaustion status, not clone numbers, correlate with clearance duration.

A novel approach reveals that HLA class 1 single antigen bead-signatures provide a means of high-accuracy pre-transplant risk assessment of acute cellular rejection

This paper develops a novel method using HLA class 1 single antigen bead signatures to predict acute cellular rejection risk pre-transplant with high accuracy.

High prevalence of S. pyogenes Cas9-specific T cell sensitization within the adult human population - A balanced effector/regulatory T cell response

The paper identifies a widespread memory/effector T cell response and regulatory T cell presence against SpCas9 in healthy humans, with implications for gene therapy.