Multiple-gene targeting and mismatch tolerance can confound analysis of genome-wide pooled CRISPR screens
Jean‐Philippe Fortin, Jenille Tan, Karen E. Gascoigne, Peter M. Haverty, William F. Forrest, Michael Rodrigues Costa, Scott E. Martin
The paper analyzes how multi-target sgRNAs in CRISPR screens introduce biases in essentiality scores and functional networks.
Genome biology · Jan 25, 2019Peer-reviewedBioinformatics