Single-Assay Characterization of Ternary Complex Assembly and Activity in Targeted Protein Degradation
Corey H. Yu, Vi Dougherty, Dongwen Lv, Pirouz Ebadi, Shikha Dubey, Digant Nayak, Anindita Nayak, Daohong Zhou, Shaun K. Olsen, Dmitri N. Ivanov
This paper introduces a fluorescent assay to quantify ubiquitination kinetics in targeted protein degradation, enabling measurement of degrader and ligase affinities and ternary complex activity.
bioRxiv · Aug 20, 2025 · PreprintBiochemistry