BiochemistrybioRxivPreprint
Single-Assay Characterization of Ternary Complex Assembly and Activity in Targeted Protein Degradation
Corey H. Yu, Vi Dougherty, Dongwen Lv…·Dmitri N. Ivanov
This paper introduces a fluorescent assay to quantify ubiquitination kinetics in targeted protein degradation, enabling measurement of degrader and ligase affinities and ternary complex activity.
DOI 10.1101/2025.08.20.671298