Shaun K. Olsen

High-throughput mapping of 6,888 RAD51D variants identifies distinct biochemical functions needed for homologous recombination and olaparib response

This paper maps 6,888 RAD51D variants to identify biochemical functions critical for homologous recombination and olaparib response, revealing key regions and mechanisms of variant impact.

Single-Assay Characterization of Ternary Complex Assembly and Activity in Targeted Protein Degradation

This paper introduces a fluorescent assay to quantify ubiquitination kinetics in targeted protein degradation, enabling measurement of degrader and ligase affinities and ternary complex activity.

Inhibition of coronaviral exonuclease activity by TRIM-mediated SUMOylation

The paper identifies TRIM32 as an antiviral protein that inhibits SARS-CoV-2 replication by SUMOylating NSP14, thereby blocking its exonuclease activity.

Distinct roles of the two BRCA2 DNA binding domains in DNA damage repair and replication fork preservation

This study identifies distinct roles of BRCA2's two DNA binding domains in DNA repair and replication fork stability using genetic and biochemical methods.

Molecular basis of interchain disulfide-bond formation in BMP-9 and BMP-10

This study investigates how BMP-9 and BMP-10 form disulfide bonds and dimers, showing that geometric strain, not cysteinylation, reduces dimerization propensity and that residue swaps can reverse this effect.

Single particle cryo-electron microscopy with an enhanced 200 kV cryo-TEM configuration achieves near-atomic resolution

This study shows that a 200 kV cryo-TEM with advanced detectors can achieve near-atomic resolution for single particle cryo-EM, comparable to 300 kV systems.