Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
D. J. Knapp, Y. S. Michaels, M. Jamilly, Q. R. Ferry, H. Barbosa, T. A. Milne, T. A. Fulga
The paper identifies sequence determinants of tRNA promoter and processing activities to engineer a Pol-II promoter for specific guide RNA expression.
bioRxiv · Jun 8, 2018Bioengineering