Disulfide chaperone knock-outs enable in-vivo double spin-labeling of an outer-membrane transporter
D. S. Cafiso, T. D. Nilaweera, D. A. Nyenhuis, R. Nakamoto
The paper shows that disulfide bond formation in E. coli prevents double spin-labeling of BtuB cysteines, but dsbA/B null mutants enable this labeling and reveal structural differences in vivo.
bioRxiv · Jul 22, 2019Biophysics