Extending conventional TIRF microscopy to image single molecules in micromolar analyte backgrounds
Riley C. Gentry, Katherine M. Leon Hernandez, Ruben L. Gonzalez, Colin D. Kinz-Thompson
This paper extends conventional TIRF microscopy to image single molecules in micromolar analyte backgrounds, enabling studies of weak biomolecular interactions.
bioRxiv · Aug 27, 2026 · PreprintBiophysics