Discovery of conformation-sensitive anti-amyloid protofibril monoclonal antibodies using an engineered chaperone-like amyloid-binding protein
W. Vallen Graham, Alessandra Bonito-Oliva, Rita Agostinelli, Riyaz Karim, Jeremy Deguzman, Kerry S. Kelleher, Marianne Petro, Anna-Karin Lindström, Caroline Graff, Kathleen M. Wood, Lioudmila Tchistiakova, Kimberly Marquette, Paul D. Wes, Thomas P. Sakmar
This paper describes a method to stabilize A{beta} protofibrils using an engineered chaperone-like protein, enabling the discovery of conformation-sensitive anti-A{beta} monoclonal antibodies that target protofibrils over monomers or plaques.
bioRxiv · Feb 24, 2019 · PreprintMolecular Biology