Efficient gene knockout and genetic interactions: the IN4MER CRISPR/Cas12a multiplex knockout platform
Nazanin Esmaeili Anvar, Chenchu Lin, Xingdi Ma, Lori Wilson, Ryan Steger, Annabel K. Sangree, Medina Colic, Sidney H. Wang, John G. Doench, Traver Hart
The paper introduces the in4mer CRISPR/Cas12a platform for efficient multiplex gene knockout, demonstrating improved sensitivity and reduced clone numbers compared to CRISPR/Cas9.
bioRxiv · Jan 3, 2023 · PreprintSystems Biology