High-content Imaging-based Pooled CRISPR Screens in Mammalian Cells
X. Yan, N. Stuurman, S. A. Ribeiro, M. E. Tanenbaum, M. A. Horlbeck, C. R. Liem, M. Jost, J. S. Weissman, R. Vale
The paper develops an imaging-based CRISPR screening method called optical enrichment to identify genes affecting nuclear size, identifying 14 hits from 6092 sgRNAs.
bioRxiv · Jun 30, 2020Cell Biology