Programmable RNA detection with CRISPR-Cas12a
S. R. Rananaware, E. K. Vesco, G. M. Shoemaker, S. S. Anekar, L. S. W. Sandoval, K. S. Meister, N. C. Macaluso, L. T. Nguyen, P. K. Jain
This paper introduces SAHARA, a CRISPR-Cas12a-based method for direct RNA detection without reverse transcription, showing improved specificity and multiplexing capabilities.
bioRxiv · Jan 29, 2023Bioengineering