Author

S. Shashkova

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 5 Biochemistry· 1 Cell Biology· 1

Signature Assays, Organisms & Methods

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Organism:yeast· 7 Research Focus:cell signaling & trafficking· 5 Assay:fluorescence / live-cell microscopy· 5 Approach:experimental assay· 4 Approach:microscopy & imaging analysis· 3 Research Focus:cell type annotation· 2 Research Focus:chromatin & epigenetic regulation· 2 Assay:proteomics· 2

Frequent Co-Authors

M. C. Leake· 6 shared A. Wollman· 3 shared S. Hohmann· 3 shared C. MacDonald· 2 shared E. Hedlund· 2 shared A. J. Wollman· 1 shared D. D. Bisinski· 1 shared J. Shepherd· 1 shared

Activity by Year

2017-2020 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 18 mutual co-author links

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6 shared papersBiophysics

M. C. Leake

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Author profile →

7 papers

Recent·Most cited

Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number

J. Shepherd, S. Lecinski, J. Wragg, S. Shashkova, C. MacDonald, M. C. Leake

This study uses FRET biosensing and microscopy to measure molecular crowding in yeast cells under varying glucose and ionic conditions, revealing uniform cytosolic crowding and sensor copy number dependencies.

bioRxiv · Aug 14, 2020Biophysics

Correlative single-molecule fluorescence barcoding of gene regulation in Saccharomyces cerevisiae

S. Shashkova, T. Nyström, M. C. Leake, A. J. Wollman

The paper uses single-molecule fluorescence microscopy to observe Mig1 transcription factor foci in yeast and correlates their patterns with growth characteristics under different carbon sources.

bioRxiv · Aug 12, 2020Biophysics

Correlating single-molecule characteristics of the yeast aquaglyceroporin Fps1 with environmental perturbations directly in living cells

S. Shashkova, M. Andersson, S. Hohmann, M. C. Leake

The paper tracks single-molecule characteristics of the yeast aquaglyceroporin Fps1 in real time under varying environmental conditions using super-resolved fluorescence microscopy.

bioRxiv · Feb 27, 2020Biophysics

A glucose starvation response governs endocytic trafficking and eisosomal retention of surface cargoes

K. M. E. Laidlaw, D. D. Bisinski, S. Shashkova, K. M. Paine, M. A. Veillon, M. C. Leake, C. MacDonald

The paper shows glucose starvation in yeast increases internalization of surface cargoes while suppressing their recycling, and upregulates eisosomal factors to aid nutrient uptake recovery.

bioRxiv · Feb 5, 2020Cell Biology

Mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells

A. Wollman, E. Hedlund, S. Shashkova, M. Leake

The paper introduces a method to map 3D genome organization in living cells using single-molecule tracking of transcription factors in budding yeast.

bioRxiv · Mar 5, 2019Biophysics

The Yeast Mig1 Transcriptional Repressor Is Dephosphorylated By Glucose-Dependent And Independent Mechanisms

S. Shashkova, A. Wollman, M. C. Leake, S. Hohmann

This study identifies both glucose-dependent and independent mechanisms of Mig1 dephosphorylation in yeast, highlighting the role of multiple phosphatases and tyrosine phosphorylation in its regulation.

bioRxiv · Apr 25, 2017Biochemistry

Transcription factor clusters regulate genes in eukaryotic cells

A. Wollman, S. Shashkova, E. Hedlund, R. Friemann, S. Hohmann, M. C. Leake

The paper shows transcription factors form clusters that regulate gene expression in yeast by reducing search times and stabilizing expression.

bioRxiv · Apr 6, 2017Biophysics