Author

S. Tschirpke

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 3

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:E. coli· 1 Assay:proteomics· 1 Organism:yeast· 1

Frequent Co-Authors

L. Laan· 3 shared W. K.-G. Daalman· 3 shared F. van Opstal· 2 shared R. van der Valk· 1 shared

Activity by Year

2023-2026 · peak 2/yr

Co-Author Collaboration Network

4 top collaborators · 6 mutual co-author links

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3 shared papersBiochemistry

L. Laan

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3 papers

Recent·Most cited

Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP

S. Tschirpke, W. K.-G. Daalman, F. van Opstal, L. Laan

This study investigates how Cdc42 GTPase cycling is regulated by the GEF Cdc24 and GAP Rga2 through oligomerization-dependent mechanisms and synergistic interactions.

EMBO Reports · Feb 9, 2026Peer-reviewedBiochemistry

Quantification of GTPase cycling rates of GTPases and GTPase : effector mixtures using GTPase Glo™ assays

S. Tschirpke, W. K.-G. Daalman, L. Laan

The paper describes the use of GTPase Glo assays to quantify GTPase cycling rates and interactions with effector proteins.

bioRxiv · Nov 24, 2023 · PreprintBiochemistry

A guide to the in vitro reconstitution of Cdc42 GTPase activity and its regulation

S. Tschirpke, F. van Opstal, R. van der Valk, W. K.-G. Daalman, L. Laan

This paper provides a guide for in vitro reconstitution of Cdc42 GTPase activity and its regulation, detailing expression, purification, and assay methods.

bioRxiv · Apr 24, 2023 · PreprintBiochemistry