W. K.-G. Daalman

Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP

This study investigates how Cdc42 GTPase cycling is regulated by the GEF Cdc24 and GAP Rga2 through oligomerization-dependent mechanisms and synergistic interactions.

Quantification of GTPase cycling rates of GTPases and GTPase : effector mixtures using GTPase Glo™ assays

The paper describes the use of GTPase Glo assays to quantify GTPase cycling rates and interactions with effector proteins.

A guide to the in vitro reconstitution of Cdc42 GTPase activity and its regulation

This paper provides a guide for in vitro reconstitution of Cdc42 GTPase activity and its regulation, detailing expression, purification, and assay methods.

Epigenetic feedback on noisy expression boosts evolvability

The paper models how epigenetic feedback on protein expression noise reduces fitness penalties and enhances evolvability by mitigating epistatic barriers in yeast.

Predicting an epistasis-rich genotype-phenotype map with a coarse-grained bottom-up model of budding yeast polarity

This paper develops a coarse-grained model of budding yeast polarity to predict genotype-phenotype relationships, revealing how epistasis influences evolutionary paths and fitness.

Adaptability and evolution of the cell polarization machinery in budding yeast

The paper investigates how the cell polarization machinery in budding yeast evolves and adapts by analyzing its resilience and redundancy through modeling, theory, and experiments.