Exploiting threonine sidechains as molecular switches to modulate the fluorescence of genetically encoded biosensors
Lee Min Leong, Sang Chul Shin, Jun Kyu Rhee, Heejung Kim, Jihye Seong, Junhyuk Woo, Kyungreem Han, Douglas A. Storace, Bradley James Baker
The paper develops a novel GEVI that brightens upon depolarization by manipulating threonine sidechains to control chromophore conformation and fluorescence transitions.
bioRxiv · Apr 25, 2022 · PreprintNeuroscience