Bradley James Baker

This paper shows how modifying the electrostatic environment of a fluorescent protein's beta-barrel can control chromophore fluorescence properties and response kinetics.

The paper uses fluorescent voltage and calcium indicators to image electrical activity in retinal ganglion cells of rd1 mice, showing cellular precision and millisecond resolution.

The paper develops a novel GEVI that brightens upon depolarization by manipulating threonine sidechains to control chromophore conformation and fluorescence transitions.

The paper develops a new family of GEVIs using intermolecular FRET, showing larger voltage-dependent signals and improved cell type specificity.

The paper identifies conserved polar residues in the voltage-sensing domain of Ciona-based GEVIs that, when mutated, shift voltage sensitivity and increase signal size.