Functional genomic screening in Komagataella phaffii enabled by high-activity CRISPR-Cas9 library
Aida Tafrishi, Varun Trivedi, Zenan Xing, Mengwan Li, Ritesh Mewalal, Sean Culter, Ian K. Blaby, Ian R. Wheeldon
The paper develops a high-activity CRISPR-Cas9 library for Komagataella phaffii and uses it to identify essential genes related to protein production, secretion, and glycosylation.
bioRxiv · Feb 8, 2024 · PreprintSystems Biology