A mobile CRISPRi collection enables genetic interaction studies for the essential genes of Escherichia coli
Kenneth Rachwalski, Megan M. Tu, Sean J. Madden, Drew Hansen, Eric D. Brown
The paper constructs a mobile CRISPRi library in E. coli to study genetic interactions of essential genes by knocking down their expression and analyzing combined effects with non-essential gene deletions.
bioRxiv · Apr 21, 2023 · PreprintSystems Biology