Author

Kenneth Rachwalski

McMaster UniversityORCID 0000-0002-2967-7811Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:drug response / drug discovery· 2 Organism:E. coli· 2 Organism:bacteria (other)· 1 Research Focus:cell signaling & trafficking· 1 Assay:CRISPR screen· 1 Research Focus:genome assembly· 1 Research Focus:host-pathogen & immune response· 1

Frequent Co-Authors

Eric D. BrowniD· 3 shared Megan M. TuiD· 3 shared A. Wei· 1 shared Alexa L. Gilberti· 1 shared Alyssa C. PollardiD· 1 shared Aya SabbahiD· 1 shared David SychanthaiD· 1 shared Dirk HackenbergeriD· 1 shared

Activity by Year

2023-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 26 mutual co-author links

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3 shared papersMicrobiology

Eric D. BrownORCID

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

A ubiquitous Streptomyces biosynthetic megacluster encodes an arsenal of synergistic biotin-targeting antibiotics

Rodion Gordzevich, Min Xu, W. Wang, Michael A. Cook, Dirk Hackenberger, Julia Patricia Deisinger, Megan M. Tu, Lindsey A. Carfrae, M. George, Kenneth Rachwalski, Kalinka K. Koteva, David Sychantha, A. Wei, Gerard D. Wright, Eric D. Brown

The paper identifies a conserved Streptomyces biosynthetic megacluster producing biotin-targeting antibiotics, effective against multidrug-resistant E. coli.

bioRxiv · Oct 9, 2025 · PreprintMicrobiology

Identifying Modulators of the Post-Antibiotic Effect

Alexa L. Gilberti, Megan M. Tu, Kenneth Rachwalski, Madeeha I. Ali, Siobhan A. Cohen, Melike Akoglu, Alyssa C. Pollard, Jinnette Tolentino Collado, Aya Sabbah, Franchesca Abou Said, Purnima Dela, Stephen G. Walker, John D. Haley, Eric D. Brown, Peter J. Tonge

This paper identifies genes that enhance the post-antibiotic effect (PAE) in E. coli using a high-throughput screen of deletion strains, revealing that rfaE deletion increases PAE by stabilizing LpxC.

ACS Infectious Diseases · Aug 6, 2025Peer-reviewedMicrobiology

A mobile CRISPRi collection enables genetic interaction studies for the essential genes of Escherichia coli

Kenneth Rachwalski, Megan M. Tu, Sean J. Madden, Drew Hansen, Eric D. Brown

The paper constructs a mobile CRISPRi library in E. coli to study genetic interactions of essential genes by knocking down their expression and analyzing combined effects with non-essential gene deletions.

bioRxiv · Apr 21, 2023 · PreprintSystems Biology