Systems BiologybioRxivPreprint
A mobile CRISPRi collection enables genetic interaction studies for the essential genes of Escherichia coli
Kenneth Rachwalski, Megan M. Tu, Sean J. Madden…·Eric D. Brown
The paper constructs a mobile CRISPRi library in E. coli to study genetic interactions of essential genes by knocking down their expression and analyzing combined effects with non-essential gene deletions.
DOI 10.1101/2023.04.21.537703