A simple cloning-free method to efficiently induce gene expression using CRISPR/Cas9
Lyujie Fang, Sandy S. C. Hung, Jennifer Yek, Layal El Wazan, Tu Nguyen, Shahnaz Khan, Shiang Yong Lim, Alex William Hewitt, Raymond C.B. Wong
This paper introduces a cloning-free CRISPR/Cas9 method to induce gene expression, showing efficient activation of neural development genes in human cells.
Molecular Therapy — Nucleic Acids · Nov 20, 2018Peer-reviewedMolecular Biology