Raymond C.B. Wong

Optimization of AAV tools to target Muller glial cells for retinal gene therapy

This study optimizes AAV serotypes and promoters to specifically target Muller glial cells in rats, identifying ShH10Y and GFAP promoter as the most effective combination for in vivo delivery.

Characterising retinal function with optomotor visual performance in P23H rodent models of retinitis pigmentosa

This study compares electrical and behavioural measures of vision in rodent models of inherited blindness, revealing that retinal dysfunction appears well before measurable vision loss.

Optimization of AAV tools to target Müller glial cells for retinal gene therapy

This study optimizes AAV serotypes and promoters to specifically target Müller glial cells in rats, identifying ShH10Y and GFAP promoter as the most effective combination for in vivo delivery.

CNVPipe: An enhanced pipeline for accurate analysis of copy number variation from whole-genome sequencing

CNVPipe improves CNV detection in WGS by integrating CNV tools with machine learning, reducing FDR and increasing sensitivity for both bulk and single-cell data.

Iso-Seq enables discovery of novel isoform variants in human retina at single cell resolution

The paper uses Iso-Seq to discover novel isoform variants in human retinal cells at single-cell resolution, identifying thousands of new transcripts and differential splicing patterns.

Transcriptomic analysis of TGFβ-mediated fibrosis in primary human Tenon's fibroblasts

This study uses RNA-seq to analyze TGFβ1-induced transcriptomic changes in human Tenon's fibroblasts, identifying differentially expressed genes and pathways involved in myofibroblast differentiation and conjunctival fibrosis.

Using RNA-targeting CRISPR-Cas13 and engineered U1 systems to reduce ABCA4 splice variants in Stargardt disease

This study evaluates RNA-targeting CRISPR-Cas13 and engineered U1 systems to reduce mis-spliced ABCA4 variants in Stargardt disease, showing significant reductions in disease-causing splice variants.

Spatial transcriptomic profiling of human retinoblastoma

This study uses spatial transcriptomics to analyze human retinoblastoma, revealing tumor heterogeneity, cell-cell communication networks, and key signaling pathways involved in tumor progression.

Knockout of AMD-associated gene POLDIP2 reduces mitochondrial superoxide in human retinal pigment epithelial cells

This study shows that knocking out POLDIP2 in human RPE cells reduces mitochondrial superoxide levels, linking POLDIP2 to oxidative stress in AMD.

Retinal aging transcriptome and cellular landscape in association with the progression of age-related macular degeneration

This study analyzes the transcriptome and cellular landscape of aging retinas in AMD patients and controls, identifying age-related genes and cellular changes associated with AMD progression.

MicroRNA-143 plays a protective role in ischemia-induced retinal neovascularization

The paper identifies miR-143 as a protective miRNA in ischemia-induced retinal neovascularization using miRNA-Seq and RNA-Seq in a rat model.

Potential modes of COVID-19 transmission from human eye revealed by single-cell atlas

The paper identifies conjunctival cells as potential entry points for SARS-CoV-2 by showing they express both ACE2 and TMPRSS2 receptors.

Functional study of the AMD-associated gene TMEM97 in retinal pigmented epithelium using CRISPR interference

This study uses CRISPR interference to show that TMEM97 knockdown protects ARPE19 cells from oxidative stress-induced death, supporting its role in AMD pathobiology.

Generation of human neural retina transcriptome atlas by single cell RNA sequencing

The paper generates a human neural retina transcriptome atlas using single-cell RNA sequencing, identifying 18 cell types and revealing a novel role for MALAT1 in rod degeneration.

Efficacy and dynamics of self-targeting CRISPR/Cas constructs for gene editing in the retina.

This study develops a self-destructing CRISPR/Cas system for retinal gene editing, showing reduced SpCas9 mRNA and improved safety without compromising on-target efficiency.

A simple cloning-free method to efficiently induce gene expression using CRISPR/Cas9

This paper introduces a cloning-free CRISPR/Cas9 method to induce gene expression, showing efficient activation of neural development genes in human cells.