Direct preparation of Cas9 ribonucleoprotein from E. coli for PCR-free seamless DNA assembly
Wenqiang Li, Shuntang Li, Jie Qiao, fei wang, Yang Liu, Ruyi He, Yi Liu, Lixin Ma
The paper describes a method to directly prepare Cas9 RNP from E. coli for PCR-free DNA assembly with high fidelity.
bioRxiv · Jun 7, 2018 · PreprintSynthetic Biology