Author

Yi Liu

Hubei UniversityORCID 0000-0002-2148-3596Unclaimed ORCID profile · follow to get notified of new preprints

Additional preprints under this name without ORCID

Yi Liu8 papers · Jilin University

Biology Disciplines

Click to filter author papers

Synthetic Biology· 2 Molecular Biology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 3 Assay:CRISPR screen· 2 Organism:E. coli· 2 Research Focus:genome assembly· 1 Research Focus:protein design· 1 Research Focus:variant calling· 1

Frequent Co-Authors

Jie QiaoiD· 3 shared Lixin MaiD· 2 shared Ruyi HeiD· 2 shared fei wang· 1 shared Junqi ZhangiD· 1 shared Qingyuan JiangiD· 1 shared Shuntang Li· 1 shared Shuqi JiniD· 1 shared

Activity by Year

2018-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 26 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

3 shared papersSynthetic Biology

Jie QiaoORCID

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

Precise amplification-free detection of highly structured RNA with an enhanced SCas12a assay

Junqi Zhang, Wenhao Yin, Qingyuan Jiang, Wuxiang Mao, Wenwen Deng, Shuqi Jin, Xinping Wang, Ruyi He, Jie Qiao, Yi Liu

This paper introduces an enhanced SCas12a assay (SCas12aV2) for amplification-free detection of highly structured RNA, showing improved sensitivity and specificity for RNA and clinical samples.

Communications Biology · Mar 4, 2025Peer-reviewedMolecular Biology

Direct purification of CRISPR/Cas ribonucleoprotein from E. coli in a single step

Siyu Lin, Jie Qiao, Lixin Ma, Yi Liu

This paper presents a method to directly produce and purify CRISPR/Cas RNP from E. coli in a single step using an ultra-high-affinity CL7/Im7 system, achieving high stability and genome editing efficiency.

bioRxiv · Jul 26, 2018 · PreprintSynthetic Biology

Direct preparation of Cas9 ribonucleoprotein from E. coli for PCR-free seamless DNA assembly

Wenqiang Li, Shuntang Li, Jie Qiao, fei wang, Yang Liu, Ruyi He, Yi Liu, Lixin Ma

The paper describes a method to directly prepare Cas9 RNP from E. coli for PCR-free DNA assembly with high fidelity.

bioRxiv · Jun 7, 2018 · PreprintSynthetic Biology