Lixin Ma

A bacterial Argonaute with efficient DNA and RNA cleavage activity guided by small DNA and RNA

This paper describes a bacterial Argonaute (MhAgo) with eight cleavage activities, capable of using all four guide types for DNA and RNA cleavage, showing high activity and diverse guide preferences.

A programmable pAgo nuclease with RNA target preference from the psychrotolerant bacteria Mucilaginibacter paludis

The paper characterizes MbpAgo, a pAgo from Mucilaginibacter paludis that preferentially cleaves RNA over DNA, and demonstrates its temperature tolerance and guide flexibility.

Direct purification of CRISPR/Cas ribonucleoprotein from E. coli in a single step

This paper presents a method to directly produce and purify CRISPR/Cas RNP from E. coli in a single step using an ultra-high-affinity CL7/Im7 system, achieving high stability and genome editing efficiency.

Direct preparation of Cas9 ribonucleoprotein from E. coli for PCR-free seamless DNA assembly

The paper describes a method to directly prepare Cas9 RNP from E. coli for PCR-free DNA assembly with high fidelity.