Orchestrated metal ion repositioning defines the dynamic catalytic strategy of the essential DNA repair nuclease APE1
Leonardo Farias Serafim, Susan Tsutakawa, Andrew S. Arvai, Bradley R. Kossmann, Anil K. Mantha, Rachel Abbotts, David M. Wilson, SANKAR P. MITRA, John A. Tainer, Ivaylo N. Ivanov
This study reveals the dynamic catalytic mechanism of APE1 using a moving metal ion strategy, showing how Mg2+ repositioning enables efficient DNA repair without forming a pentavalent intermediate.
bioRxiv · May 14, 2026 · PreprintBiochemistry