Detection of precisely edited CRISPR/Cas9 alleles through cointroduced restriction-fragment length polymorphisms
Chon‐Hwa Tsai‐Morris, Sydney Hertafeld, Yvonne V Rosario, James Iben, Eric Chang, Ling Yi, Steven L. Coon, Stephen Gerard Kaler, Ryan Dale, Benjamin Feldman
The paper introduces TIARS, a method to detect precise CRISPR/Cas9 edits using restriction-fragment length polymorphisms, and validates it in zebrafish for atp7a gene mutations.
bioRxiv · Apr 20, 2021 · PreprintGenetics