Large ribosomal subunit, eIF5B, Met-tRNAiMet and mRNA cooperate to complete accurate initiation.
J. Wang, J. Wang, B.-s. Shin, T. Dever, J. Puglisi, I. Fernandez
The paper determines the cryoEM structure of a long-lived translation initiation intermediate involving eIF5B, Met-tRNAiMet, and the ribosome post GTP hydrolysis.
bioRxiv · May 30, 2020Biochemistry