Author

J. Puglisi

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Biochemistry· 3

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 2 Research Focus:cell signaling & trafficking· 1 Assay:cryo-EM· 1 Organism:human· 1 Assay:imaging· 1 Approach:microscopy & imaging analysis· 1 Organism:mouse· 1 Research Focus:protein structure prediction· 1

Frequent Co-Authors

A. Petrov· 1 shared A. Prabhakar· 1 shared A. Scaiola· 1 shared B.-s. Shin· 1 shared C. Lapointe· 1 shared E. Baeumlin· 1 shared E. D. Karousis· 1 shared E. Kummerant· 1 shared

Activity by Year

2020-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

1 shared paperBiochemistry

A. Petrov

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

Universal features of Nsp1-mediated translational shutdown by coronaviruses

K. Schubert, E. D. Karousis, I. Ban, C. Lapointe, M. Leibundgut, E. Baeumlin, E. Kummerant, A. Scaiola, T. Schoenhut, J. Ziegelmueller, J. Puglisi, O. Muehlemann, N. Ban

This study investigates Nsp1-mediated translational shutdown in three Betacoronaviruses, revealing a conserved mechanism and identifying how Nsp1 allows viral mRNA translation.

bioRxiv · May 31, 2023Biochemistry

Partial spontaneous intersubunit rotations in actively translating ribosomes

T. Huang, J. Choi, A. Prabhakar, J. Puglisi, A. Petrov

This study uses high-speed single-molecule TIRF microscopy to show that actively translating ribosomes undergo partial spontaneous rotations between three states, bridging conflicting models of ribosome dynamics.

bioRxiv · Dec 23, 2020 · PreprintBiochemistry

Large ribosomal subunit, eIF5B, Met-tRNAiMet and mRNA cooperate to complete accurate initiation.

J. Wang, J. Wang, B.-s. Shin, T. Dever, J. Puglisi, I. Fernandez

The paper determines the cryoEM structure of a long-lived translation initiation intermediate involving eIF5B, Met-tRNAiMet, and the ribosome post GTP hydrolysis.

bioRxiv · May 30, 2020Biochemistry