Q4ddPCR (May the Fourth Be Precise): A Flexible, 4-Target Assay for High-Resolution HIV Reservoir Profiling
Rachel Scheck, Mark Melzer, Gregory T. Gladkov, Adam R. Ward, Daniel B. Reeves, Naomi Perkins, T. Thinh Huynh, Deborah K. McMahon, Ronald J. Bosch, Bernard J. C. Macatangay, Joshua C. Cyktor, Joseph J. Eron, John W. Mellors, Rajesh Tim Gandhi, Lisa Buchauer, , Christian Gaebler
Q4ddPCR improves HIV reservoir quantification by targeting four conserved regions, achieving 95% accuracy and revealing clonal dynamics not detectable by IPDA.
bioRxiv · Jul 28, 2025 · PreprintImmunology