A CRISPR/Cas9-based system using dual-sgRNAs for efficient gene deletion in Mycobacterium abscessus
Linai Li, Dan Wang, Xin Li, Yuxiang Hu, Shengjuan Bao, Taibing Deng, Qinglan Wang
This paper develops a CRISPR/Cas9 system with dual-sgRNAs for efficient large-fragment gene deletion in Mycobacterium abscessus, achieving over 90% deletion efficiency at certain loci.
Frontiers in Microbiology · Jul 9, 2025Peer-reviewedMicrobiology