Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAs
Yang Zhang, Tuan M. Nguyen, Xiao-Ou Zhang, Limei Wang, Tin Phan, John G. Clohessy, Pier Paolo Pandolfi
This study compares shRNA and CRISPR/Cas13d screens for circRNA function, finding that optimized CRISPR/Cas13d reduces false positives and identifies true functional circRNAs.
Genome biology · Jan 21, 2021Peer-reviewedMolecular Biology