Direct Measurement of Light and Heavy Antibody Chains Using Differential Ion Mobility Spectrometry and Middle-Down Mass Spectrometry
R. D. Melani, K. Srzentic, V. R. Gerbasi, J. P. McGee, R. Huguet, L. Fornelli, N. L. Kelleher
This paper introduces a rapid FAIMS tandem MS method for characterizing monoclonal antibody light and heavy chains, reducing analysis time by 40-60% compared to LC-MS/MS.
bioRxiv · Jul 5, 2019Biochemistry