Genome-scale metabolic rewiring to achieve predictable titers rates and yield of a non-native product at scale
D. Banerjee, T. T. Eng, A. K. Lau, B. Wang, Y. Sasaki, R. A. Herbert, Y. Chen, Y. Liu, J.-P. Prahl, V. R. Singan, D. Tanjore, C. J. Petzold, J. D. Keasling, A. Mukhopadhyay
This study engineers Pseudomonas putida to produce indigoidine with high titer, rate, and yield by coupling product formation with growth through metabolic rewiring and CRISPRi knockdowns.
bioRxiv · Feb 21, 2020Bioengineering