Metabolically robust autoluminescent reporters
Viktor V. Morozov, Anastasia V. Balakireva, Feng Gao, Aubin Fleiss, Natalia M. Mishina, Maxim M. Perfilov, Tatiana Yu Mitiouchkina, Dmitry A. Gorbachev, Keith V. Wood, Ilia V. Yampolsky, Karen S. Sarkisyan, Alexander S. Mishin
This paper introduces a method to make autoluminescent reporters robust to metabolic changes by using a reference luciferase variant to quantify gene expression independently of metabolism.
bioRxiv · Aug 4, 2025 · PreprintPlant Biology