bioRxivPlant BiologyPreprint
Non-invasive imaging of salicylic and jasmonic acid activities in planta
Anastasia V. BalakirevaiD, Tatiana A. KarataevaiD, Michael KarampeliasiD, Tatiana Yu MitiouchkinaiD, Jan Machacek, Ekaterina S. ShakhovaiD, Maxim M. PerfiloviD, Olga A. BelozerovaiD, Kseniia A. PalkinaiD, Nikola Drazna, Zuzana VondrákováiD, Aubin FleissiD, Liliia I. FakhranurovaiD, Nadezhda M. MarkinaiD, Viktor V. Morozov, Evgenia N. Bugaeva, Galina M Delnova, Vladimir V. ChoobiD, Ilia V. YampolskyiD, Jan PetrášekiD, Alexander S. MishiniD, Karen S. SarkisyaniD
Abstract
Jasmonic and salicylic acids are the major hormones involved in plant response to pests and pathogens. Here, we engineered autoluminescent plants that report activity of these hormones with up to 53-fold contrast. Using consumer-grade cameras, we imaged reporter Arabidopsis thaliana and Nicotiana benthamiana plants throughout normal development, and in response to attacks of pests and pathogens.
- organism: Arabidopsis
- organism: mouse
- assay: imaging
- research focus: cell type annotation
- approach: experimental assay
- Citations
- 3
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