Alexander S. Mishin

Metabolically robust autoluminescent reporters

This paper introduces a method to make autoluminescent reporters robust to metabolic changes by using a reference luciferase variant to quantify gene expression independently of metabolism.

Visualization and Prediction of in vivo Phosphate Dynamics via Auto-Glowing Plant Sensors

This paper develops a biosensor to visualize and predict phosphorus deficiency in plants using bioluminescence and deep learning.

The two key substitutions in the chromophore environment of mKate2 to produce an enhanced FusionRed-like red fluorescent protein

The paper describes a new red fluorescent protein variant that outperforms its parents in brightness, fluorescence lifetime, and blinking properties.

Developing a versatile technology for agroinfiltration in multiple plants

The paper develops a fungal bioluminescence pathway-based reporter system to improve agroinfiltration efficiency across multiple plant species.

Non-invasive imaging of salicylic and jasmonic acid activities in planta

The paper develops autoluminescent plants to non-invasively image jasmonic and salicylic acid activities in response to pests and pathogens.

A platform for lab management, note-keeping and automation

The paper describes a no-code lab management platform using Airtable for integrated sample, procedure, and data management with custom automations.

Heterogeneity of the GFP fitness landscape and data-driven protein design

The paper characterizes fitness landscapes of fluorescent proteins and finds that flat landscapes are less suitable for machine learning-based protein design.

Suppression of liquid-liquid phase separation by 1,6-hexanediol partially compromises the 3D genome organization in living cells

The paper investigates how 1,6-hexanediol modulates liquid-liquid phase separation and its impact on 3D chromatin organization in living cells.

Experimental assay of a fitness landscape on a macroevolutionary scale

The paper experimentally assays a fitness landscape on a macroevolutionary scale by analyzing the effects of amino acid substitutions in yeast His3 orthologs.